使用杭州欣友生物技术有限公司产品的文献

• Structural cell biology by mega-expansion microscopy
  I Vega-Vásquez, OI García-Martínez - bioRxiv 2026
  … 1.5H, Cellvis C1-1.5HN) or 24mm cover glass coverslips (thickness No. 1.5H, Marienfeld, 0117640) for imaging. Coverslips or Cellvis were previously cleaned via four …
• Programmable protein reference standards for benchmarking sub-10-nm fluorescence microscopy
  M Budiarta, D Helmerich, M Streit, M Jungblut, S Doose - bioRxiv 2026
  … 447 Fully expanded samples were cut to size then mounted on APTES- or PDL-coated 1-well 448 chambered slides (Cellvis, #C1-1.5HN) for SRRF imaging. 449 …
• Protocol to define the in situ proteome of endogenous PRC2 bodies in the triple-negative breast cancer cell line BoM-1833 using optoproteomics
  YC Pan, HJ Chang, WM Chong, JC Liao, C Lungu - STAR protocols 2026
  Polycomb repressive complex 2 (PRC2) and its catalytic subunit EZH2 regulate transcriptional repression and display subcellular organization patterns important for cell …
• PHF19 drives the formation of PRC2 clusters to enhance motility in TNBC cells
  N Pelzer, T Lukic, W Ge, N Schnabel, P Teufel - Cell Reports 2025
  … To prepare the samples, approximately 4.5 × 105 cells were seeded at 80% confluency in a one-well chamber slide (C1-1.5HN, Cellvis, USA). For each biological replicate…
• QuantiTrack: A unified software to study protein dynamics in living cells
  DA Ball, K Wagh, DA Stavreva, L Hoang, RL Schiltz - … 2026
  … 100,000 MCF-7 cells expressing Halo-GR or H2B-Halo were plated in 2 well chamber slides (Cellvis #C2–1.5HN) in charcoal-stripped FBS supplemented medium for 24 …
• Benchmarking three simple DNA staining-based image metrics for live-cell tracking of chromatin organization
  M Kang, AT Cabral, M Sawant, HR Thiam - bioRxiv 2026
  … plated on a non-coated, gamma-irradiated 238 glass-bottom 35 mm dish (World Precision Instruments, FD35-100) or 4-well chambered 239 coverglass (CellVis, C4-1.5HN…
• Multidomain Coupling Governs FoxP1 Assembly and Nuclear Compartmentalization.
  AF Lazaro-Alfaro, J Avilés, TO Peulen - Biorxiv: the Preprint … 2026
  … CC-BY 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in …
• Nanoscale Fluorescent Characterizations and Origin Tracing of Extracellular Vesicles through Standard Preparation Methods
  HDW Herath, H Jing, M Li, JW Franses, YS Hu - ACS nano 2026
  … Eight well-chambered cover glass (C8–1-N) was purchased from Cellvis. Unconjugated gold colloids (15711–20) were … bioRxiv , 2025. DOI: 10.1101/2025.08.28.672797 …
• A minimal kinase-phosphatase system and its lipid substrates self-organize into dynamic patterns
  TS Hsieh, B Ramm, Q Yu, JL Yuly, J Pfister, Y Wu - bioRxiv 2026
  … Mammalian cell transfection and imaging HeLa cells were seeded on 8-well chambered coverglass (C8-1.5HN, CellVis, Mountain View, CA) at a density of approximately …
• Programmable protein reference standards for benchmarking sub-10-nm fluorescence microscopy
  M Budiarta, D Helmerich, M Streit, M Jungblut, S Doose - bioRxiv 2026
  … DNA-PAINT Single-Molecule Surface Preparation: The surfaces of 8-well chambered cover 381 glass with high performance cover glass (Cellvis, C8-1.5HN) were washed …
• Quantitative Motion-Corrected PALM Links Endosome Structure and Dynamics in Live Cells
  Y Xu, S Adhikari, EM Puchner - bioRxiv 2026
  … Eight-well chambered borosilicate coverglasses (C8-1.5HN; Cellvis) were prepared by incubating each well with 100 µL of 0.8 mg/mL sterile Concanavalin A (ConA) for …
• Artificial endoplasmic reticulum-lipid droplet tethers facilitate lipid incorporation into lipid droplets
  VH Williams, GE Miner, S Cohen - bioRxiv 2026
  Lipid droplets (LDs) are ubiquitous organelles that store neutral lipids to meet cellular energetic and signaling needs. As a unique monolayer structure, LDs arise from the …
• A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment
  N Acosta, R Gong, Y Su, J Frederick, K Medina - Journal of Visualized … 2026
  … Eight well chambered cover glass Cellvis C8-1.5HN Can be any glass bottom plate, but volumes must be adjusted … bioRxiv: The Preprint Server for Biology. , (2024). …
• An engineered streptavidin condensate platform for chemically inducible control of endogenous proteins in mammalian cells
  T Kamikawa, CJ Wilson, I Lan, Y Nihongaki - bioRxiv 2026
  … , who has granted bioRxiv a license to display the preprint in perpetuity. It is made … (which was not certified by peer review) is the author/funder, who has granted bioRxiv a …
• Transcription condensates are promoter hubs that enhance transcriptional bursts
  G Pandey, J Galeota-Sprung, A Budhathoki - … 2026
  … Cells were seeded onto either 35 mm glass-bottom dishes (Celvis D35-20-1.5H) or 8-well 690 chambered coverglass slides (Celvis C8-1.5HN). The imaging dishes were …
• Naked antisense oligonucleotides remain endolysosomally sequestered despite induced membrane damage.
  E Sitarska, A Saminathan, G Scanavachi - Biorxiv: the Preprint … 2026
  Antisense oligonucleotides (ASOs) enter cells efficiently, but the compartment from which 37 productive escape occurs remains uncertain. We used live-cell microscopy, …
• Targeting cancer-associated cell surface RNAs with oligonucleotide-drug conjugates enables broad antitumor activity
  C Luo, C Cheng, X Zhao, J Zhou, Z Jing, Q Ma, L Ye - bioRxiv 2026
  … CC-BY-NC-ND 4.0 International license made available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display …
• Hypervariable loop profiling decodes sequence determinants of antibody stability
  Y Wan, J Liang, Y Dai, K Srinivasan - Nature Structural & … 2026
  Antibody folding and aggregation are major challenges in the development of relevant reagents and therapeutics. Antibodies face a biophysical trade-off; the immense …
• Dietary intake and BCAA metabolism regulate pulmonary fibrosis through KDM4A-mediated epigenetic remodeling in male mice
  J Yao, S Fang, M Lei, Z Ou, C Zeng - Nature … 2026
  … Cells were seeded onto eight-chamber glass-bottom dishes (Cellvis, C8-1.5HN) and fixed in 4% paraformaldehyde (Biosharp, BL539A) for 10 min at room temperature …
• Selective Editing and Functionalization of the Mammalian Lipidome
  B Wang, L Lüthy, L Tenney, L Qi, T Harayama - … 2026
  … HeLa cells were seeded in 8-well chamber with glass imaging slide (Cellvis #C8–1.5HN) 24h before imaging experiment in DMEM (10% FBS, 1% P/S), such that cells …
• The COX2-PGE2-PKA Axis Suppresses Antiviral Immunity by Inhibiting mtDNA-Dependent STING Activation
  PTT Vo, J Cicero, Z Wang, H Hakozaki, TS Hoang - … 2026
  … Cells were imaged in eight-well chamber slides (C8–1.5HN, Cellvis). To quantify extramitochondrial DNA, cells were labelled with the following dyes: PicoGreen (1:500, …
• Fatty acids in the tumor microenvironment reprogram neutrophils to induce immunosuppression via adenosine
  R Singhal, NW Zhang, ZH Lee, HN Bell, PJ Dalal - … 2026
  … Bone marrow-derived neutrophils were seeded at 2 × 10 cells per well in 8-well glass-bottom chamber slides (Cellvis, #C8-1.5HN), precoated with 25 μg/ml fibrinogen and …
• Polyglutamine homorepeat regulates Runx2 condensation and cellular localization in a KPNA3-dependent manner
  JH Weng, SB Xu, JC Lv, YY Li, H Chen, TR Zhang - Cell Reports 2026
  … The protein solution was loaded onto an 8-well chamber (Cellvis, C8-1.5HN) for 5 … MC3T3-E1 or C2C12 cells were seeded onto 8-well chamber slides (Cellvis, C8-1.5HN). …
• Membrane Tension Integrates Physical and Signaling Cues to Gate Cell Fate Transitions
  G Ali, D Gibbard, E Ghelfi, A Olson, J Cai, A Balagtas - … 2026
  … 8-well #1.5 coverglass chambers (Cellvis, C8–1.5HN), pre-coated with growth factor … well #1.5 cover glass chambers (Cellvis, C8–1.5HN). Cells were maintained at 37C in …
• Impact of Docking Strand Design on Spatial Resolution in DNA‐Points Accumulation for Imaging in Nanoscale Topography
  DA Helmerich, M Budiarta, P Eiring, M Sauer - … 2026
  … The surfaces of 8-well chambered cover glass with high performance cover glass (Cellvis, C8-1.5HN) were washed once with double distilled water (ddH2O) and treated …
• Programmable and Switchable RNA Scaffolds for Synthetic Condensate Engineering in Mammalian Cells
  Z Xue, O Mukherjee, L Mi, Y Guo, R Zheng, C Yuan - … 2026
  … NanoLuc luminescence imaging, HEK293T cells were seeded at 3 × 10 4 cells per well in poly-D-lysine-coated 8-well chambered coverglass plates (Cellvis, C8–1.5HN) …
• Divergent condensates tune transcriptional responses during stress
  JN Dudley, JE Berends, K De Chayan, T He - … 2026
  … , siCtrl or siHSF1 transfected cells were trypsinized from 6-well plates 24 h after transfection and reseeded into either 8 well chambered cover glass (Cellvis, C8–1.5HN) or …
• Nup42 safeguards heat-induced mRNAs from nuclear condensation to support chaperone synthesis
  E Tassoni-Tsuchida, A Madero, M Zaoralová - … 2026
  … After heat shock, cells were centrifuged and concentrated to 100 μL, and 50 μL was added to concanavalin-A treated wells in microscopy chambers (Cellvis, #C8–1.5HN), …
• Fatty Acid Oxidation Suppression Reprograms Fibroblasts in Fibrostenotic Crohn's Disease
  J Aljabban, A Awad, BD McMichael, V Gartner - bioRxiv 2026
  … For lipid droplet quantification, human intestinal myofibroblasts were seeded at 1,000 cells per 701 well into 8-well chambered coverglass slides (Cellvis, Cat# C8-1.5P) …
• Preferential formation of NUP98-KDM5A condensates at specific H3K4me3-rich loci drives leukemogenic gene expression
  A Berrocal, JE Sandoval, N Khetan, A Ma, T Wang - … 2026
  … was performed as follows: 15 000–25 000 cells were plated into each of the wells of a Cellvis 8-chambered coverglass system (#1.5 polymer coverslip; Product#: C8-1.5P) …
• SIMBA: an agentic AI platform for single-molecule multidimensional imaging
  H Mao, H Mauny, O KanchanadeviVenkataraman - … 2026
  … The cells were seeded in an 8-well chambered cover glass (Cellvis, C8SB-1.5H) with 50–70% confluency. After 24 hours of seeding, the cells were proceeded with dye …
• Far-red-emitting Nile red with boosted solvatochromism enables multidimensional imaging of membrane heterogeneity and dynamics
  M Liu, Y Fu, X Li, H Zhang, Y Ma, M Li, C Shan - The Innovation 2026
  … When the cells reached 80% confluence, they were detached using trypsin-EDTA and subsequently seeded onto 8-chambered cover glasses (Cellvis, C8SB-1.5H) for …
• 2-Hydroxyglutarate Redirects Fatty Acid Partitioning to Mitigate Lipotoxic Stress and Preserve Metabolic Fuel
  N Vigder, A Chandra, N Shrimali, S Tumanov, V Elgart - bioRxiv 2026
  … (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this …
• Chromatin architectures underlying plasmid-based assays for regulatory variant effects
  BJ Mallory, TW Tullius, CG Biar, CP Herlihy - Molecular Cell 2026
  Plasmids have fundamentally transformed how we resolve regulatory grammar across the tree of life. However, our understanding of how, or whether, chromatin structures …
• Selective Editing and Functionalization of the Mammalian Lipidome
  B Wang, L Lüthy, L Tenney, L Qi, T Harayama - … 2026
  … The complete version history of this preprint is available at bioRxiv. … HeLa cells were seeded in 18-well Cellvis 18 Chambered Coverglass System (C18–1.5H), coated with …
• Lineage tracing reveals atoh7 positive and negative retinal ganglion cell populations in the zebrafish retina
  DM Bennett, RI Newland, MB Veldman, JB Miesfeld - bioRxiv 2026
  … The complete version history of this preprint is available at bioRxiv. … no SDS) overnight at 37C in the dark, using an 18 Chambered Coverglass System (Cellvis, C18–1.5H). …
• mRNAbow: A versatile gene expression system for multiplexed fluorescent imaging using optimized in vitro transcribed mRNA
  H Choi, C Halanych, W Kasberg, MD Testa - bioRxiv 2026
  … the cells for transfection and imaging, iPSC-CMs at D30 after differentiation will be reseeded in Matrigel (Corning) -precoated 18 well CellVis chamber (CellVis, C18-1.5H) …
• FERONIA kinase and PP2A antagonistically regulate salt tolerance in Arabidopsis
  J Liu, M Wang, X Liu, X Wang, Z Li, J Luo, W Lin - Current Biology 2026
  Leucine-rich repeat extensin (LRX) 3/4/5 and FERONIA (FER) jointly regulate plant salt tolerance, but the mechanisms by which LRX3/4/5 modulate FER activity in …
• Stress granule component protein TIA1 is a negative regulator of the noncanonical NLRP3 inflammasome
  PP Lamichhane, Aditi, BH Neil, PB Kilgore - … 2025
  … BMDMs were seeded at a density of 80,000 cells per well in 18-well chambered cover glass (C18-1.5H, Cellvis). After overnight incubation, the cells were washed with …
• A genetic screen for modifiers of cohesin clustering identifies regulators of genome folding
  W Kim, DS Park, SC Nguyen, R Yang, EF Joyce - Science … 2026
  … C18SB- 1.5H) were coated with poly- d- lysine. siRNA was mixed with RNAiMAX … C18SB- 1.5H) and treated with 1 μM auxin for 3 days. Cells were then fixed and …
• abcFISH enables multiplexed, single-molecule visualization of circular RNA spatial heterogeneity
  YX Liu, BW Jiang, KM Liu, L Yang, LL Chen - BioRxiv 2025
  Functional RNAs often exhibit distinct subcellular localization, but studying circular RNA (circRNA) localization has been challenging due to their extensive sequence …
• A Novel Hydrogen Persulfide Donor HPD1 Alleviated LPS-Induced Neuroinflammation​ by Modulating Glial Polarization Throughtlr4-MAPK/NF-Κb Pathway
  F Yan, S Tao, Y Jiao, T Kang, S Du, Y Cheng, Z Huang - papers.ssrn.com
  The role of hydrogen persulfide (H2S2) has remained elusive due to the scarcity of controllable donors, despite its importance as a reactive sulfur species whose …
• Chaperonin TRiC bridges radial spokes for folding locally translated proteins to sustain mammalian sperm flagellar motility
  X Meng, L Li, C Lin, Y Zhu, Y Feng, Q Zhao, N Zhao - Molecular Cell 2026
  As animals evolved from external to internal fertilization, sperm flagella, once transiently propelling sperm in water to reach nearby eggs, developed to beat for days in the …
• Mitochondrial-derived vesicles drive budding-type fission of damaged lysosomes
  Y Luo, J Yu, Z Li, W Li, L Jiang, C Huang, Z Rong - Nature Cell … 2026
  Lysosomes are central degradative organelles essential for cellular homeostasis, yet the mechanisms that maintain their integrity and function under stress remain …
• Single-Molecule Localization Microscopy in the Nucleus with Self-Labelling Tags: Halo, SNAP, and CLIP Tags
  AJ McCann, FA Meunier - Super-Resolution Microscopy: Methods and … 2026
  … D29-20-1.5 N for microscope stage inserts that suit 29 mm glass-bottom dishes, and Matek Cat. No. P35-1.520C for microscope stage inserts that suit 35 mm glass-bottom …
• Organization of Myosin H in the Apical Complex of Toxoplasma Gondii Revealed by 3D Single-Molecule Super-Resolution Microscopy
  A Balaji, LS Zarko, AES Barentine, JC Boothroyd - … 2026
  … Tachyzoites were placed into imaging chambers (Cellvis, D29–20-1.5H) precoated with poly-L-lysine and centrifuged to facilitate attachment to the glass bottom. Samples …
• The Erlin1/2 complex is a dynamic scaffold for membrane microdomain assembly on the endoplasmic reticulum
  L Yan, Z Xu, Y Yao, T Awang, X Wang, Y Wang, C Ma - Molecular Cell 2026
  … Immunofluorescence staining and STED imaging analysis U2-OS cells were cultured on 29 mm glass-bottom dishes (Cellvis, D29-20-1.5H) for treatment and …
• Method for modeling oviduct function and impact on embryonic development
  KK Stephens, V Ahmad, MA Silva, MK Shifflett, J Mao - bioRxiv 2026
  … (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this …
• Protocol for scalable generation of uniform 3D porcine oviduct epithelial cell spheroids
  LM Molina, HC Fan, AM Antonson, DJ Miller - bioRxiv 2026
  … CC-BY 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in …
• Ultra-structural analysis of mineralized extracellular matrix in osteogenic monolayers and spheroids: comparison of sample preparation methods
  D Boscaro, U Ludacka, P Sikorski - bioRxiv 2026
  … D35-10-1.5-N), covered with a GN-6 Metricel® 0.45 µm-47 mm sterile membrane and gelation was induced by adding 50 mM CaCl2 on top of the membrane for 5 minutes…
• Mechanoresponsive modulation of nuclear pore complex structure and function by O-GlcNAc
  S Chandra, KJ Morgan, WL Chadwick, MC King - bioRxiv 2026
  … Cells expressing GFP or mCherry-based reporters were grown on 35 mm glass bottom dishes (D35-10-1.5-N; CellVis) and incubated with medium lacking phenol-red …
• Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs
  S Maruyama, CF Henderson, N Swinhoe - Cell 2026
  Endosymbiosis has spurred the evolution of new organelles across life. Corals and other cnidarians have repeatedly evolved an organelle, called the symbiosome, which …
• Modeling the cell biology of PEX11β deficiency during human neurogenesis
  C Bodnya, RP Theart, V Gama - bioRxiv 2026
  … Neural progenitors were generated from iPSCs as described above and plated onto Matrigel-coated 35mm imaging dishes (Cellvis #D35–10-1.5-N). At day 3 of neural …
• Label-free imaging of matrix mineralization in alginate-encapsulated bone spheroids using Coherent Raman Scattering microscopy
  D Boscaro, SJ Kjeldgaard-Nintemann - … & Biomedical Imaging 2026
  Three-dimensional (3D) cell cultures, such as spheroids, are increasingly used to perform advanced studies on bone matrix mineralization. However, their full …
• Acute in vivo proximity labeling for membrane targeted proteomics in neuronal circuits
  M Anguiano, R Zhang, M Robles, KP Adams - bioRxiv 2025
  … Neurons were plated on treated 35 mm glass bottom dishes (D35-14-1-N, Cellvis) and maintained at 37C, 5% CO2. The culture media was replaced 24 hr after the plating …
• Revealing the spatiotemporal dynamics of methionine metabolism with a genetically encoded single-fluorophore biosensor
  KA Cohen, A Jhawar, G Garcia, N Goni, M Chen - bioRxiv 2026
  … Cells were seeded in 35 mm glass bottom dishes (Cellvis, Cat# d35-14-1.5-n). Transfections for HeLa and U2OS were conducted one day prior to imaging using 500 ng …
• α-Synuclein triggers intercellular nanotubes formation to prevent apoptosis in astroglia by promoting stemness
  R Kashayap, A Sreenivas BK, V MR, RR Mundada - bioRxiv 2026
  … pre-coated with Poly-D-Lysine or in glass-bottom 35 mm dishes (Cellvis, D35-14-1.5-N) … density of 60,000 cells in glass-bottom 35mm dishes (Cellvis, #D35-14-1.5-N) and …
• Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes
  JV Bogdanović, J Galeota-Sprung, AS Kainth - bioRxiv 2026
  … (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this …
• Biomechanical Isolation is Required for Maintenance of the Cardiac Pacemaker Cell Fate
  M Bressan, A Laidman, T Henley, W Shi, K Scherrer - 2026 2026
  … Explants were placed in 35mm glass bottom dishes (Cellvis, D35-14-1.5-N) for culture at 37C in 2mL complete media … bioRxiv preprint doi: https://doiorg/101101/824185. …
• Direct Membrane Penetration of Oligoarginines by Fluorescence and Cryo-electron Microscopy Combined with Molecular Simulations
  KL Baxová, MI Morandi, N Scher, P Kula, O Tichacek - bioRxiv 2026
  … The grids were left in a tissue culture hood under UV for 1 hour before being transferred into a 14 mm glass-bottomed well of a 35 mm imaging plate (D35-14-1.5N, Cellvis)…
• Denatured Albumin Gains a Function of Regulating Platelet Activity
  V Pandey, S Kundu, SJ Wang, A Pyne, L Que, X Wang - bioRxiv 2026
  … 200 µL casein solution was added to the glass surface of a glass-bottomed Petri-dish (D35-14-1.5-N, Cellvis) which was subsequently incubated for 30 minutes at room …
• Steric shielding of the KRAS4B hypervariable region enables isoform-specific inhibition of prenylation
  JN Maskos, Y Stark, VL Rohner, A Häfliger - bioRxiv 2026
  … The cultures were maintained at 37 C with 5% CO₂ in an imaging dish with a glass bottom (D35-14-1.5-N, Cellvis). One hour before injection, the medium was replaced …
• Mechanical Signaling Drives Tunneling Nanotubes to Preserve Cytoskeleton Tension and Lamin Integrity Against α-Synuclein-Induced Senescence in Astroglia
  S Chatterjee, A Ravula, A Sreenivas BK, A Raghavan - bioRxiv 2026
  … https://doi.org/10.64898/2026.03.13.711517 doi: bioRxiv preprint … Glass-bottom 35 mm dishes (Cellvis, D35-14-1.5-N) were employed for certain experiments. …
• Red-Shifted Chemigenetic Glutamate Indicators for Recording Multiplexed Neural Computation
  N Tjahjono, JY Min, JA Chouinard - Available at SSRN …
  Neural computation relies on the integration of neurotransmitters, neuromodulators, and intracellular signal transduction. However, direct measurement of these …
• Digest before Ingest: Early Recruitment of Membrane-bound DNaseX to Phagocytic Cups in Macrophages
  A Pyne, V Pandey, S Kundu, S Ikegami, X Wang - bioRxiv 2026
  … 400 µL bacterial solution was incubated on glass bottom 475 petridish (D35-14-1.5-N, Cellvis) at room temperature condition for 1-3 days. The biofilm thickness depends …
• An ER retention motif controls the heteromeric stoichiometry of hERG1a/1b channels
  S Kannan, LR Ernandez, GA Robertson - bioRxiv 2026
  … The complete version history of this preprint is available at bioRxiv. … bottom dishes with #1.5 cover glass (Cellvis, D35-14-1.5-N) using DMEM culture media (ThermoFisher, …
• Preparation of samples for Lipid-CLEM imaging
  A Nadler, HM Lennartz - 2026 2026
  … Under the cell culture hood: Place Sapphire disks in 35 mm glass bottom dishes Cellvis, #D35141.5N) and pre-incubate with cell culture medium for 30 min at 37 C. …
• Tissue-scale mechanics controls differentiation strategy and dynamics of epithelial multilayering
  C Villeneuve, SAE Hassikpezi, M Albu, M Rübsam - bioRxiv 2026
  Generating and maintaining multilayered epithelia requires coordinated cell division, differentiation, and tissue architecture, yet the precise mechanisms of multilayering …
• LKB1 Spatially Regulates AMPK Activity Coordinate Cellular Oxidative Stress Response
  K Parks, A Jhawar, A Andrikopoulos, DM Winters - bioRxiv 2025
  … (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this …
• Imaging the time course of DNA damage response at a nonrepetitive endogenous locus
  AT Rybczynski, WT Cottle, PT Chen, J Kwon - Cell Reports … 2025
  … Etoposide treatment U2OS cells were plated in the center of 35 mm dishes containing 14 mm glass-bottom microwell inserts (Cellvis, d35-14-1.5-N) at a density of 100,000 …
• Substrate-dependent cytoskeletal changes and particle internalization in U2OS osteosarcoma cells
  C RzÄ…ca - 2026 2026
  … step, 22 µL of the solution was placed in the 35 mm glass bottom dishes with 20 mm well, and a thickness of #0, designed for high-resolution imaging (Cellvis, D35-200-N). …
• TLR7 Signaling Regulates Embryonic Hematopoietic Stem Cell Development by Sensing microRNA-146a in Vertebrates
  H Liu, K Zhou, K Zhu, YF Li, L Mo, PF Xu, Y Li - bioRxiv 2026
  The specification of hematopoietic stem cells (HSCs) is tightly regulated by multiple transcription factors and signaling pathways. Inflammatory signaling is pivotal for …
• Calcium Signaling in Macrophages During a Wound Response In Vivo
  JA Munos, PY Lam - International Journal of Molecular Sciences 2026
  … D35-20-0-N) using 1% low melt agarose (LMA; Genesee Scientific (El Cajon, CA, USA), cat no. 20-104 or GoldBio, cat no. A-204… bioRxiv 2018. [Google Scholar] [CrossRef] …
• Epithelial Collective Response to Immune Cells, Matrix Proteins, and Mechanical Cues
  HM Zmuda - 2025 2025
  Epithelial cells form complex and dynamic tissue structures within the human body. During these processes, cells must come in contact with a variety of dissimilar cell types…
• Cooperative antibiotic response in coupled biofilm and planktonic E. faecalis communities.
  GF Martins, KA Guardiola-Flores, L Zaman - Biorxiv: the Preprint … 2026
  … A 276 total of 2.7 ml of each mixture was transferred to 35 mm glass bottom microscope dishes (Cellvis, 277 D35-20-1-N) and incubated at 37C. Samples were kept …
• SLIRP maintains energy metabolism homeostasis in colorectal cancer by stabilizing mitochondrial-encoded mRNAs: Cellular and Molecular Biology
  C Yang, Y Ming, Q Wu, Z Ye, Y Duan, H Ma - British Journal of … 2026
  … Cells were seeded into confocal dishes (Bio-Thera Solutions, #D35-20-1-N) and incubated with pre-warmed DMEM containing 500nM MitoTracker Deep Red (Thermo …
• ASFV p54 manipulates the secretory carrier membrane protein 3-dependent apoptotic bodies pathway to enhance cell-cell transmission
  Y Li, D Di, J Liu, C Tian, R Wang, Z Xie, J Gao, M Wang - Cell Reports 2026
  The African swine fever virus (ASFV) is a significant threat to the global swine industry, with no effective vaccine available. ASFV subverts cellular processes, including …
• Modern Manufacturing, Characterization, and Remediation Strategies for Engineered and Environmental Nanoparticles
  R Chairil - 2025 2025
  … The dish is a Cellvis D35-20-1-N which has a 35 mm … dish (Cellvis catalog number D35-20-1-N) and consists of a 2 … The dish is a Cellvis D35-20-1-N which has a 35 mm …
• Atypical signaling, ligand recognition and selective agonist discovery of complement receptor C5aR2
  J Qin, C Cai, M Shan, S Zhou, Q Shen, T Zhu, M Zhao - Cell Research 2026
  C5a, the most potent anaphylatoxin in the complement system, exerts its effects through the canonical G protein-coupled receptor C5aR1 and the arrestin-coupled …
• Arp2/3-mediated turnover of large clathrin lattices is regulated through the tyrosine kinase ACK
  MA Hazelbaker, DJ Michalak, MT Butler, JR Beach - bioRxiv 2026
  … For high-resolution TIRF and confocal imaging, glass bottom dishes (Cellvis, Cat#: D3520-1.5N) were first coated with 10 µg/ml fibronectin (Corning, Cat#: 356008) or 2% …
• Instant Prior-Free Resolution Enhancement for Cross-Modality Microscopy
  H Gan, S Peng, H Hu, X You, Y Guo, R Guo, Z Chen - bioRxiv 2026
  … bottom dishes (Cellvis, D35-20-1.5-N) for 24 h before being stained with 200 nM PK Mito Orange in serum-free DMEM (30 min, 37C, 5% CO₂). After two washes, the …
• ATL2 recruits TRAK1 to promote mitochondrial transport at ER–mitochondria contact sites
  Y Cheng, P Chai, X Pei, Y Chen, X Huang - Advanced … 2026
  … For live-cell imaging, COS-7 cells in glass-bottom dishes (Cellvis, D35-20-1.5-N) were incubated with 125 nM PK Mito Deep Red (Genvivo, PKMDR-2) at 37◦C for 45 min …
• Tumor-Associated EDA-FN-Enriched Matrix Instructs Macrophage Behavior
  G Bashiri, E Bakare, J Longstreth, MS Padilla, K Wang - bioRxiv 2026
  … 35-mm dishes with a 20-mm glass-bottom inset (CellVis, D35-20-1.5-N) were treated with 1N H2SO4 for 20 min, followed by three washes with deionized water and air …
• The proximal lipid phase of PI3K signaling is confined to the plasma membrane
  MMC Ricci, H Patel, MJ Montoya, KL Jayasuriya - bioRxiv 2026
  … Scientific, 50-153-2238) placed in the wells for immunofluorescence and the 35-mm circumferential, 20mm #1.5 optical glass bottom dishes (CellVis, D35-20-1.5-N) used …
• STREMI: a dual-function upstream ORF-encoded regulator of mitochondrial cristae architecture
  R Guo, Y Guo, R Shu, J Qian, J Wang, R Li, T Qin - EMBO reports 2026
  … HeLa cells were cultured in 35-mm glass-bottom dishes (CELLVIS, D35-20-1.5-N) overnight. After washing by PBS once, cells were fixed by 4% PFA (Solarbio, P1110) at …
• EPS8 dampens the growth dynamics and prolongs the lifetime of actin-based protrusions
  AG Mulligan, ZJ Lehmann, KL Robinson, MJ Tyska - bioRxiv 2026
  … Cells were incubated in Lipofectamine and transfected with 100 ng - 2.2 μg total of DNA, overnight in 35mm glass-bottom dishes (Cellvis #D35–20–1.5-N), 4-chamber 35 …
• ER-associated control of axonal vesicle trafficking during neuronal development
  M Subra, JB Meehl, RG Abrisch, GK Voeltz - bioRxiv 2026
  … Neurons were grown on 35 mm imaging dishes (Cellvis, D35-20-1.5-N) and transfected with the indicated plasmids using Lipofectamine 2000 (Thermo Fisher Scientific, …
• Ratiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity
  S Hario, N Tamura, BS Alladin-Mustan, SM Ali - bioRxiv 2026
  … CC-BY-NC-ND 4.0 International license made available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display …
• Biomolecular condensates provide a unique environment for redox-mediated protein crosslinking
  H Wang, B Favetta, J Wang, C Hoffmann, E Maloku - … 2026
  … Experiments were conducted in glass-bottom dishes (D35-20-1.5-N, Cellvis). Prior to MPA, optical tweezers-assisted fusion was performed to generate large condensates (…
• Microtubules in the axon are GDP bound but adopt a stable GTP-like expanded state
  EA Zehr, S Sun, SL Sarbanes - Nature Structural & … 2026
  … After three washes with ddH2O, grids were placed into a 35-mm glass-bottom Petri dish (In Vitro Scientific, D35-20-1.5-N) and coated with Matrigel for 15 min at 37 C. …
• Actin-membrane interface stress regulates Arp2/3-branched actin density during lamellipodial protrusion
  MT Butler, MA Hockenberry, HH Truscott, WR Legant - … 2026
  … The complete version history of this preprint is available at bioRxiv. … D35–20-1.5-N) were coated with 0.01% poly-L-Lysine solution (Millipore Sigma, cat. no. P4707) or …
• Membrane-protein-mediated phase separation orchestrates organelle contact sites
  C Hoffmann, T Nagao, TA Tsunoyama, JV Tromm - Molecular cell 2026
  Mitochondria and the endoplasmic reticulum (ER) contain large areas that are in close proximity. Yet the mechanism of how these inter-organellar adhesions are formed …
• DEAD-box ATPase–marked condensates coordinate compartmentalized translation and antibiotic persistence
  Z Zhang, D Li, B Zheng, J Liu - Science Advances 2026
  … The LB medium gel pad was prepared as a gel island at the center of a confocal dish (Cellvis, D35-20-1.5-N). Cells were monitored under differential interference contrast (…
• Biophysics of Biomolecular Condensate Interfaces: From Designed Proteins to SARS-CoV-2
  B Favetta - 2025 2025
  … The MPA experiments were conducted in glass-bottom dishes (D35-20-1.5-N, Cellvis), under brightfield illumination to minimize potential artifacts associated with …
• The actin crosslinker Fimbrin is required for force sensing at tricellular junctions
  N Taneja, CA Looney, MF Moubarak, MJ McGovern - Developmental Cell 2026
  … Transfected cells were seeded onto 35 mm dishes with a 20 mm glass bottom coverslip (CellVis D35-20-1.5H) precoated with 0.01% poly-L-lysine (Sigma) and allowed to …
• Studium role HDAC1 a HDAC3 v reakci na poÅ¡kození lidského a myšího genomu UV zářením
  B Plecáková - 2026 2026
  … Následně byla transfekční směs nakapána na misky se sklíčky o půměru 35 mm (Glass bottom dishes, Cellvis, #D35-20-1.5H), na kterou byly den předem nasazeny buňky …
• Transcription condensates are promoter hubs that enhance transcriptional bursts
  G Pandey, J Galeota-Sprung, A Budhathoki - … 2026
  … Cells were seeded onto either 35 mm glass-bottom dishes (Celvis D35-20-1.5H) or 8-well 690 chambered coverglass slides (Celvis C8-1.5HN). The imaging dishes were …
• Fluorescence anisotropy structured illumination microscopy for quantitative super-resolved mapping of cell microenvironment and cytoskeletal dynamics
  S Gao, W Wang, L Qiao, H Wang, M Liu, Y Hou, G Xin - bioRxiv 2026
  … Cells were plated on the 35-mm glass-bottom dishes (Cellvis, #1.5H, D35-20-1.5H) and grown 682 typically overnight in full culture medium to reach ~50% confluency (~70…
• Programmable and Switchable RNA Scaffolds for Synthetic Condensate Engineering in Mammalian Cells
  Z Xue, O Mukherjee, L Mi, Y Guo, R Zheng, C Yuan - … 2026
  … For transfection, cells were seeded at a density of 3.2 × 10 5 cells per 35-mm poly-D-lysine-coated glass-bottom dish (Cellvis, D35–20-1.5H) and cultured overnight. …
• Dynamic s-acylation of schizophrenia-linked antioxidants explicate the redox flexibility of human microglia during inflammation
  S Mukherjee, A Sharma, P Upadhyay, E Madan, S Pati - bioRxiv 2026
  … Aβ-treated cells (2.5*104 cells) were either sub-cultured in 35mm dishes (cellvis, # D35-20-1.5P) for confocal-microscopic live cell imaging, or in a six-well plate (with 1*…
• Simple and Multiplexed Tracking of Bacterial Growth in Double Emulsion Droplets
  S Somolinos Cedeño, S Thompson, P Fordyce, D Endy - bioRxiv 2026
  … To prototype a simple implementation of a trap-less imaging set-up, we used a glass bottom dish (D35C4-20-0-N, Cellvis) and increased the relative volume of the outer …
• A Commensal-Derived Lipoteichoic Acid Engages an Inducible Neuronal PD-1 Checkpoint to Suppress Inflammatory Pain
  Z Liu, YH Cheng, CV Osborn, M Martina, AJ Schaeffer - bioRxiv 2026
  Background Immune checkpoint receptors regulate adaptive immunity but are increasingly recognized as modulators of neuroimmune interactions. The upstream signals …
• Myofibroblasts slow down defect recombination dynamics in mixed cell monolayers
  Z Zheng, Y Luo, J Chen, Y Luo - arXiv preprint arXiv:2512.22923 2025
  Cellular organization and mechanotransduction pathways are crucial regulators of tissue morphogenesis, whereas their dysregulation contributes to pathologies. …
• Subtype-resolved norepinephrine imaging with an engineered α1D-adrenergic receptor indicator
  VL Rohner, Z Kagiampaki, P Bohne, AR Klein - Cell Reports … 2026
  … seeded into 35 mm glass bottom dishes (D35C4-20-1-N; Cellvis) and transfected at a … into 35 mm glass bottom dishes (D35C420-1-N; Cellvis) 24 h prior to the experiment. …
• Subnuclear cofactor partitioning underlies auxin-dependent transcriptional regulation
  J Hernandez-Garcia, M Dipp-Alvarez, M de Roij - bioRxiv 2026
  … Cells were seeded in 35 mm imaging dishes (Cellvis, D35C4-20-1-N) a day prior to transfection. At approximately 70% confluency, cells were transfected with 250 ng of …
• The Disordered Region of ASXL1 Acts as an Auto‐Regulator Through Condensation
  X Fang, Q Li, W Zhang - Advanced Science 2026
  Intrinsically disordered regions (IDRs) are common in chromatin regulators, yet how their sequence encodes regulatory logic remains unclear. Here, we show that the long …
• Aneuploidy selects for the acquisition of driver genes in breast cancer
  KN Al-Zahrani, ER Langille, J Nurtanto, A Obersterescu - Nature 2026
  Chromosome instability is highly prevalent in cancer and drives large-scale chromosomal imbalances, known as aneuploidies 1 , 2 , 3 – 4 . How aneuploidy contributes to …
• Circadian temperature compensation is intrinsically linked with metabolism and redox signaling
  Y Xu, T Mori, H Mehra, A Ustione, K Tanaka - bioRxiv 2026
  … Mammalian U2OS cells were seeded into 4-sector-glass bottom dishes (D35C4-20-1.5-N, Cellvis) and cultured with DMEM + 10 % fetal bovine serum, and 100 IU mL-1 …
• LRRK2 mutations block NCOA4 trafficking upon iron overload leading to ferroptotic death
  A Goldman, M Nguyen, J Lanoix, C Li - Journal of Cell … 2026
  … For live imaging approximately 100,000 cells were seeded in glass bottom imaging plates (Cellvis D35C4-20-1.5-N) and stained using normal growing media …
• Activating adaptor-like sequences in pericentrin mediate its transport by dynein
  W Zhang, AG Sangster, TT Nguyen, S Melancon - bioRxiv 2026
  … CC-BY 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in …
• HTRA1 deficiency in COL4A1 mutant hiPSC-derived astrocytes: a convergent mechanism of cerebral small vessel disease
  X Qi, A Granata, T Van Agtmael, S Sinha, MZ Cader - bioRxiv 2026
  … Four-compartment glass-bottom chamber slides (Cellvis, D35C4-20-1.5-N) were precoated with Matrigel at a concentration of 8.5 μg/cm2 per compartment and incubated …
• EPS8 dampens the growth dynamics and prolongs the lifetime of actin-based protrusions
  AG Mulligan, ZJ Lehmann, KL Robinson, MJ Tyska - bioRxiv 2026
  … with 100 ng - 2.2 μg total of DNA, overnight in 35mm glass-bottom dishes (Cellvis #D35–20–1.5-N), 4-chamber 35 mm glass-bottom dishes (Cellvis #D35C4–20-1.5-N), or …
• RNA polymerase loss by nuclear rupture drives LMNA cardiomyopathy
  A En, M Gucwa, E Rapushi, C Barnett, W Katano - bioRxiv 2026
  … ), 1:100 penicillin–streptomycin (Invitrogen, 105727), 25 µM Cytochalasin D, and 20 mM HEPES (pH 7.4), and plated onto glass-bottom dishes (MatTek D35C4-20-1.5-N) …
• Red-Shifted Chemigenetic Glutamate Indicators for Recording Multiplexed Neural Computation
  N Tjahjono, JY Min, JA Chouinard - Available at SSRN …
  Neural computation relies on the integration of neurotransmitters, neuromodulators, and intracellular signal transduction. However, direct measurement of these …
• A compact, portable degron tool derived from the 3′UTR of MTCH2 for tunable degradation of proteins
  LE Manjunath, S Som, R Boro, A Dhar, Eepsitha - bioRxiv 2026
  Targeted protein degradation relies on short sequence elements, termed degrons, that direct proteins to the cellular degradation machinery. Here, we report a nine-amino-…
• LRRK2G2019S acts as a dominant interfering mutant in the context of iron overload
  A Goldman, M Nguyen, J Lanoix, A Fahmy, Y Zhong Xu - bioRxiv 2025
  … For live imaging approximately 100.000 cells were seeded in glass bottom imaging plates 538 (Cellvis D35C4-20-1.5-N) and stained using normal growing media …
• Isotropic shrinkage of patterned vacancies enables three-dimensional nanoprecise metastructures for visible light applications
  Q Yang, G Yang, T Nambara, H Kusaka, Y Kunai - Nature … 2026
  … After washing with ddH2O, the hydrogels were cut into ~1.5 × 1.5 cm squares, transferred into a glass-bottom dish (D60-30-1-N; Cellvis) and immersed in ~5 ml of …
• Microscale spatial fragmentation promotes bacterial survival under antibiotics
  D Benbenisi, T Orevi, GS Hamrick, L You, N Kashtan - bioRxiv 2026
  Most bacteria inhabit fragmented microscale environments rather than well-mixed systems in which antibiotic efficacy is typically defined. Here, we show that microscale …
• Understanding the Control Logic of Subcellular Self-Organization: From Ciliate Morphology to Biomolecular Condensates
  ZM Xu - 2026 2026
  Biological systems self-organize internal components into functional structures across scales through the dynamic interplay between intracellular regulatory networks and …
• Serotonin Receptor Signaling Promotes Amoeboid Migration of Melanoma Cells Through cAMP/PKA Activation
  AG Abrenica, S Kuang, N Kar, JS Logue - bioRxiv 2026
  … CC-BY 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in …
• Systematic functional genetic analysis of cell-substrate adhesion
  K Manzer, KC Su, MM Di Bernardo, HR Keys, R Walton - bioRxiv 2026
  … KO HeLa cell library was induced with 1 μg/mL doxycycline hyclate for 78 hours and seeded at a density of 350k cells/well in 6-well glassbottom plates (Cellvis, P06-1.5HN…
• Cell. Author manuscript; available in PMC 2020 October 17. Published in final edited form as: Cell. 2019 October 17; 179 (3): 787–799. e17. doi: 10.1016/j. cell …
  D Feldman, A Singh, JL Schmid-Burgk, RJ Carlson - dspace.mit.edu
  polymerase, 0.5 U/μL Ampligase and 50 nM dNTPs) for 5 minutes at 37C and 90 minutes at 45C, and then washed 2 times with PBS-T. Circularized padlocks were …
• Revealing the spatiotemporal dynamics of methionine metabolism with a genetically encoded single-fluorophore biosensor
  KA Cohen, A Jhawar, G Garcia, N Goni, M Chen - bioRxiv 2026
  … Cells were seeded and grown to 70-80% confluency in 6-well glass bottom dishes (Cellvis Cat #P06-1.5HN) prior to treatment with small molecules. Following treatment, …
• Leptin drives osteoarthritis pain through sensory neuron reprogramming independent of cartilage signaling
  HD Welhaven, EK Truchan, KL Lenz, BA Andoko - bioRxiv 2026
  … The cells were then plated on 6 well glass bottom plates (P06-1.5HN) precoated with 0.1mg/mL of poly-D-lysine (Sigma-Aldrich, P7405-5MG). The cultures were placed in …
• Scalable multimodal mapping of macrophage regulatory architecture by integrating optical and transcriptomic pooled screens
  T Kudo, R Lopez, AM Meireles, A Rios, P Coelho - bioRxiv 2026
  Understanding how genetic perturbations reshape cellular states requires measuring diverse phenotypic modalities at scale. Here, we present PerturbPair, a platform that …
• A Far-Red FRET biosensor for AMPK enables multiplexed imaging of single-cell bioenergetic homeostasis.
  NL DeCuzzi, M Hardy, MB Cabel, J Hu - Biorxiv: the Preprint … 2026
  … (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this …
• A unified photosensitizer platform for in situ DNA-, RNA-, and protein-directed proximity labeling
  EB Biletch, CP Herlihy, L Li, M Krebs, CJ Kelly - … 2026
  … For each ISH-POCA experiment, 300,000 cells were seeded into each well of a glass-bottom 6-well plate (Cellvis P06-1.5HN) to culture for 24 hours to reach 750,000 - 1 …
• The one-week automated genome-wide optical pooled screen
  B Kirby, M Di Bernardo, IM Cheeseman, PC Blainey - bioRxiv 2026
  … a 24-hour incubation, cells were washed once with 1X PBS, passaged with TrypLE (Thermo Fisher Scientific 12604013), and seeded in two six-well plates (P06-1.5HN) at …
• A defined 2D system for generating and expanding human basal radial glia from iPSCs
  A Artioli, M Gasparotto, AC Rossetti, Y Hass - bioRxiv 2026
  … For live imaging of IST events, bRG cells were plated onto 6-well glass bottom plates (IBL P06-1.5HN), GT-coated and imaged on a Nikon TI2-E inverted video-microscope …
• Quantitative live imaging of junctional neural tube formation
  J Wang - 2026 2026
  … The embryos were then mounted with mounting media (Sigma-Aldrich, F4680-25ML) on a six-well glass bottom plate (Cellvis, P061.5HN) for imaging or stored at 4oC in …
• Long-range actin-driven endosymbiont mobility in an animal-algal symbiosis
  G Zhong, G Toullec, PH Jouneau - Available at SSRN … 2026
  … For Waminoa sp., worms were placed in glass bottom dishes (Cellvis, P06-1.5HN), and allowed 384 to settle on the microscope for 30 minutes up to a few hours without …
• 2050-084X
  KY Tony, W Luke, J Laura, ES John - eLife
  … Unless otherwise specified, black glass-bottom 6-well plates (P06-1.5HN, Cellvis) were used for microplate imaging. Prior to imaging, the FLS media was replaced with …
• Structural Mechanisms of DNAJC13 Dimeric Assembly and InsP6 binding in Recycling Endosome Regulation
  T Fu, C Lee, FV Hundley, JA Paulo, JW Harper - bioRxiv 2026
  … P12-1.5HN) and dried overnight in a chemical fume hood to form a uniform film. Lipid mixtures were prepared in chloroform at a total stock concentration of 1 mg/mL. …
• Systematic functional genetic analysis of cell-substrate adhesion
  K Manzer, KC Su, MM Di Bernardo, HR Keys, R Walton - bioRxiv 2026
  … with 10% FBS, 100 U/mL penicillin and streptomycin, and 2 mM L-glutamine and plated onto fibronectin-coated wells in a glass-bottom 12-well plate (Cellvis; P12-1.5HN). …
• Ribozyme-optimized CVS-N2cΔG Rabies Virus with Hyper-diverse Barcodes for High-fidelity Single‑Neuron Projection Connectivity
  L Jin, H Zhang, D Liu, X Gao, X Ge, Y Li, X Zhang - 2026 2026
  Neural circuit mapping at single‑cell resolution relies on hyper‑diverse barcode libraries to minimize collision artifacts and topological distortion. Here, we report a …
• RIG-I-like receptors sense replication stress through ribosomal DNA integrity to drive inflammation
  IH Wu, X Wang, R Clark, S Farahnak, S Ahmad, S Hur - bioRxiv 2026
  … IRF3–GFP RPE-1 ∆p53 cells were seeded in 12-well glassboUom plates (Cellvis, P12-1.5HN) and imaged under the indicated conditions using a Nikon Ti inverted …
• CRISPR activation of endogenous PKD1 increases polycystin-1 levels and suppresses cellular features of ADPKD
  A Chakraborty, MM Varghese, DP Wallace, CJ Ward - … 2026
  … /- cells were nucleofected with CRISPRa reagents and 72 h post-nucleofection, cells were detached and reseeded into 12-well glass-bottom plates (Cellvis, P12-1.5HN) to …
• Ribophorin-1 Governs Spike Abundance of Highly Pathogenic Coronaviruses by ER-associated degradation
  YJ Huang, L Lv, W Ran, H Zhao, YQ Deng, Q Ye, L Li - bioRxiv 2026
  … For live-cell imaging, cells were seeded in 12-well glass-bottom plates (Cellvis, P12-1.5HN). At 6 h post-transfection, time-lapse imaging was initiated using a Zeiss …
• scGeneScope: A Treatment-Matched Single Cell Imaging and Transcriptomics Dataset and Benchmark for Treatment Response Modeling
  J Dapello, M Nassar, R Eksi, B Wang - Advances in … 2026
  … Cells were plated in 12-well glass bottom plates (CellVis #P12-1.5HN) 24 hours before the start of treatment in exact duplicate plates. Compounds were added to the wells …
• Genetically encoded manipulation of ATP/ADP ratio in human cells uncovers proteomic and physiological signatures of energy stress
  AE Ekvik, MM Kober, DV Titov - Cell Chemical Biology 2026
  … with the doxycycline-inducible ATPGobble or LOF constructs, as well as cpmVenus or PercevalHR, were seeded at 150K per well on a 12-well plate (Cellvis P12-1.5HN), …
• Nuclear Biophysical Properties are Altered During Herpes Simplex Virus 1 Infection, Increasing Fluidization and Enabling Condensate Formation
  NL Herzog - 2026 2026
  Molecular processes are profoundly influenced by the biophysical properties of the cell interior. However, the mechanisms controlling these physical properties and the …
• Subcellular Characterization of the Molecular Determinants of Ebola VP40 Trafficking and Assembly
  T Huth, E Wiggenhorn, S Khanal, W Wan - bioRxiv 2026
  … For confocal imaging, Vero E6 cells were seeded in glass bottom 12-well plates (Cellvis P12–1.5HN) at approximately 70,000 cells / well to obtain 60–80% confluency 24-…
• Epithelial Collective Response to Immune Cells, Matrix Proteins, and Mechanical Cues
  HM Zmuda - 2025 2025
  Epithelial cells form complex and dynamic tissue structures within the human body. During these processes, cells must come in contact with a variety of dissimilar cell types…
• Regulation and Function of the Hidden Proteome in Mammalian Cells
  J Ly - 2026 2026
  The human body is composed of trillions of cells and exhibits remarkable biological complexity—far exceeding that of single-celled organisms like yeast or bacteria. Yet …
• Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model
  E Skoufa, J Zhong, K Hu, O Kahre, G Tsissios - Science … 2025
  … For immunostaining in 2D cultures, the induction protocol was performed in 12- well glass- like polymer coverslip- bottom plates (Cellvis P12- 1.5P). On day 7, samples …
• Human neuromodulatory assembloids to study serotonin signaling and disease
  S Kanton, X Meng, C Dong, F Birey, D Wang, N Reis - … 2026
  … Assembloids were transferred to a well in a 24-well glass bottom plate (Cellvis, P24–0-N) in culture medium, and images were taken using a 10x objective to capture the …
• Microtubule detyrosination enhances matrix remodeling and force transmission during endothelial sprouting
  D Mohammed, I Hamid, S Steffens, C Lemeslier - bioRxiv 2026
  … P24-0-N), and allowed to polymerize at 24 37 C for 10 min. For imaging chambers, 40,000 HUVECs per chamber were seeded 25 to obtain a confluent monolayer by …
• A human iPSC-based neural spheroid platform for modelling glioblastoma infiltration using high-content imaging
  VSK Tsang, F Riccio, AS Wilson, H Nudds - Scientific Reports 2025
  Glioblastoma is the most aggressive adult brain tumour, characterised by resistance to therapy and high recurrence due to diffuse infiltration. We developed a …
• Structural Mechanisms of DNAJC13 Dimeric Assembly and InsP6 binding in Recycling Endosome Regulation
  T Fu, C Lee, FV Hundley, JA Paulo, JW Harper - bioRxiv 2026
  … CC-BY 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in …
• Organ identity shapes autophagy dynamics and selectivity in plants
  AN Dauphinee, S Holla, S Mazumdar, FIM Ballhaus - bioRxiv 2026
  … BioRxiv. Organ identity shapes autophagy dynamics and selectivity in plants … or 4-well glass bottom plates (P24-1.5HN, Cellvis), and treated with 5 µM AZD and 0.5 µM …
• A systems-level proteomic analysis identifies kinesin targets of KIFBP during neuronal development
  SC Paschall, TL Blasius, A Missman, P Rodriguez - bioRxiv 2026
  … captured at 20X magnification in 483 widefield mode in a 5 X 5 image montage in the center of the well of the fixed 24 well glass 484 bottom plate (Cellvis; P24-1.5HN). …
• Tubulin autoregulation mediator TTC5 regulates neuronal morphology and migration
  SL Sarbanes, J Ziak, AL Kolodkin, A Roll-Mecak - bioRxiv 2026
  Microtubule dynamics regulation is critical for neuronal development, yet how neurons regulate tubulin levels remains poorly understood. Tetratricopeptide repeat domain …
• Opposing roles of serine and charge in IDR condensate miscibility
  G Pei, X Wang, X Quan, D Geng, Z Chen, W Xu - Nature Chemical … 2026
  … For imaging, cells were grown in 24-well glass-bottom plates (Cellvis, P24-1.5HN) and imaged on a Nikon A1R HD25 confocal microscope at 37 C/5% CO2. Blue light (…
• LASER couples damage sensing to ESCRT assembly for lysosome repair
  CS Goul, A Jain, S Yitiz, ZE Soltani, S Yang, S Rapp - Nature 2026
  … For i3 neurons: At day 4 post-differentiation, i3 neurons plated in 24-well glass bottom plates (CellVis, P24-1.5HN) were transduced with concentrated mCherry–Gal3 or …
• A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo.
  S Yang, AJ McDonald, X Lu, V Villette - Biorxiv: the Preprint … 2026
  … Before 759 seeding the hippocampal neurons, a 24-well glass-bottom plate (P24-1.5HN, Cellvis) was coated with 760 Neuron Coating Solution (027-05, Sigma-Aldrich) at …
• Embryo-scale Visual Cell Sorting reveals a conserved transcriptomic signature of nucleolar size linked to proteostasis
  HJ Kim, S Pendyala, J Lin, T Cooke, G Li, WS Noble - bioRxiv 2026
  … experiment, U2-OS cells stably expressing either H3-GFP or H3-miRFP were co-cultured at a density of 100k per well in a glass-bottom 24 well plate (Cellvis, P24-1.5HN). …
• Microbial cross-feeding interactions reshape evolutionary trajectories of consumers by preserving motility
  T Rosazza, Z Al-Tameemi, A Rodríguez-Verdugo - bioRxiv 2026
  … putida (GFP) in co-culture, we used a 444 glass-bottom 24-well plate with #1.5 cover glass (Cellvis P24-1.5HN). Wells were pre- 445 filled with 1960 mL of FAB medium …
• Single Treatment of Mature 3D Single-, Dual-and Poly-Species Biofilms Using a Combination Therapy of Phage or Phage-Hetero-Cocktails and Ciprofloxacin
  T Glonti, MK Aktan, C Cochez, N Zayed, A Braem - Antibiotics 2026
  Background/Objectives: Biofilms are a form of defense that enables bacteria to withstand antibiotic pressure and demonstrate antibiotic resistance. It is crucial to develop …
• Integrative vasculogenesis unifies distinct endothelial sources in the developing lung
  M Meziane, MPH Litz, P Chandrasekaran, D Frank, P Li - bioRxiv 2026
  … Explants for live imaging were plated in CellVis 24-well glass bottom imaging plates (CellVis, P24-1.5HN) coated with rat tail collagen (Life Technologies, A1048301). …
• Targeting cancer-associated cell surface RNAs with oligonucleotide-drug conjugates enables broad antitumor activity
  C Luo, C Cheng, X Zhao, J Zhou, Z Jing, Q Ma, L Ye - bioRxiv 2026
  … Chemicals and enzymes are listed as names (vendor, catalog number): Glass bottom 24-well plates (Cellvis, P24-1.5HN). 8-well chambered cover glass (Cellvis, C8-1.5…
• Human Sperm-Induced Cell-Cell Fusion Requiring JUNO (hSPICER): A paradigm shift to test sperm fertilizing potential
  NG Brukman, M Kabha, R Levi, S Baram - bioRxiv 2026
  … P24-1.5HN) pre-treated with 20 μg/ml of Poly-L-lysine and transfected with 1 µg pCI::H2B-RFP or pCI::hJUNO::H2B-RFP. Alternatively, HEK293T (DSP1-7 or DSP8-11) …
• ATG9A-mediated plasma membrane repair is linked to Vps13A and regulated by glycosylation
  NH Muskat, I Nevo-Yassaf, M Chaurasia - Proceedings of the … 2026
  … Cells were plated onto coverslips in 24-well plates or on 24-well black plates with glass bottoms (Cellvis, P24-1.5HN). Cells were transfected with plasmids and treated as …
• Designer indicators for two-photon recording of subthreshold voltage dynamics
  MA Land, M Galdamez, V Villette, J Zhu, X Lu - Nature … 2026
  Subthreshold voltage dynamics are essential for neuronal information integration, yet they remain technically challenging to measure in vivo. While genetically encoded …
• A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo
  AJ McDonald, MA Land, S Yang, N Hakam, V Villette - … 2026
  … Before seeding the hippocampal neurons, a 24-well glass-bottom plate (P24–1.5HN, Cellvis) was coated with Neuron Coating Solution (027–05, Sigma-Aldrich) at 37 C …
• Synthetic budding morphogenesis by optogenetic receptor tyrosine kinase signaling
  LS Prahl, R Canlla, AZ Huang, DS Alber, SL Shefter - … 2026
  … For 3D iUB branching experiments, wells in black-walled 24-well plates (#1.5H coverslip, P24-1.5HN, Cellvis) were pre-coated with 10 μL GFR Matrigel and allowed to …
• A calcium imaging pipeline to detect and quantify compound-specific effects in human and mouse astrocytes and astrocyte-neuron cocultures
  J Krohn, L Breuer, S Wegmann, C Dean - bioRxiv 2026
  … effects in human and mouse astrocytes and astrocyte-neuron cocultures | bioRxiv … glass bottom well plates (24-well (Cellvis, P24-1.5HN): ∼52,000 cells/cm², 500 µL …
• Cysteines are critical determinants of spontaneous and seeded tau aggregation in cells
  P Jayan, S Rastogi, V Bommareddy - Research … 2026
  … Cells were transduced for 48 hours and then harvested, and 15,000 cells were replated in a fibronectin-coated 24-well coated glass-bottom plate (Cellvis #P24-1.5HN). …
• TROP2 confers resistance to oxidative stress-induced cancer cell death through YAP/HMOX1 signaling
  D Wu, Y Liu, T Zhu, H Zhao, W Cao, X Guo - Journal of Translational … 2026
  … , cells were seeded onto a glass bottomed 24 well plate (Cellvis, P24-1.5 HN) … bioRxiv [Preprint]. 2024:2024.12.02.626446. 12. Mohajan S, Jaiswal PK, Vatanmakarian M, …
• Uncovering cancer dependencies in peptide-interacting protein pockets
  M Örd, L Kogan, D Bradburn, M Leiser, M Kõivomägi - europepmc.org
  … (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this …
• Uncovering cancer dependencies in peptide-interacting protein pockets
  M Örd, L Kogan, D Bradburn, M Leiser, M Kõivomägi - … 2026
  … HCT116 cells transduced with HES1 WRPW-GFP expression constructs were grown to 30% confluency in a Cellvis P24-1.5P 24-well plate with polymer coverslip base (…
• Benchmarking Machine Learning and Automated Image Analysis for Organelle Quantification
  C Daul, P Tournier, SJ Habib - Chemical & Biomedical Imaging 2026
  … For all experiments, the cells were seeded at a density of 2.6 × 10 3 cells/cm 2 in Cellvis 24-well imaging bottom plates (P24-1.5P) and cultured for 48 h prior to imaging, …
• Toward an all-in-one recombinant adeno-associated virus vector for functionally ablating the prion gene using CRISPR-Cas technology
  C Verkuyl, A Belotserkovsky, T Zerbes, D Williams - Plos one 2025
  … 1x105 cells were seeded on a 24 well glass imaging plate (catalog number P24-1.5P, Cellvis, Mountain View, CA, USA) and proliferated to ~70–80% confluence prior to …
• Mitotic DNA repair by TMEJ suppresses replication stress-induced nuclear envelope reassembly defect
  G Ye, Y He, Y Zhang, D Li, F Liu, Y Li, Q Ge - Nature … 2025
  Replication stress (RS), if not effectively and timely addressed, could result in DNA damage in mitosis. However, the relationship between RS and other mitotic events, …
• Mechanisms of Tau Proteostasis Across Development and Neurodegeneration
  MR Shin - 2026 2026
  Developing human neurons express abundant tau—including phospho-epitopes associated with Alzheimer’s disease (AD)—yet remain resilient to tau-induced toxicity. …
• Cinnamic‐Hydroxamic‐Acid Derivatives Exhibit Antibiotic, Anti‐Biofilm, and Supercoiling Relaxation Properties by Targeting Bacterial Nucleoid‐Associated Protein …
  H Chen, Y Xu, Z Xiong, H Wang, X Wang - Advanced … 2026
  … Biofilms were allowed to form in a 96-well glass bottom plate (Cellvis, P960-N). 200 μL of … Liu, bioRxiv 2025, 2025. [60] JLN Dinglasan, H. Otani, DT Doering, D. …
• A human iPSC-based neural spheroid platform for modelling glioblastoma infiltration using high-content imaging
  VSK Tsang, F Riccio, AS Wilson, H Nudds - Scientific Reports 2025
  Glioblastoma is the most aggressive adult brain tumour, characterised by resistance to therapy and high recurrence due to diffuse infiltration. We developed a …
• Adaptive Suppression of MAPT Transcription Maintains Tau Proteostasis in Developing Human Neurons
  MR Shin, N Firouzshahi, G Liddiard, M Weis - Research … 2025
  … P96–0-N) for 1 hour, protected from light at 37 C. A Bio-Tek plate reader was used to measure proteasome activity, measuring fluorescence activity (ex380 nm and em460 …
• Impaired lipoprotein secretion by APOE4 leads to lysosomal and mitochondrial dysfunction in human microglia
  JS Revanna, K Wessendorf-Rodriguez, Q Xiao - bioRxiv 2026
  … iMG were plated at a density of 70K cells/well in a glass bottom black-walled 96well plate (Cellvis, P96-1-N) coated with Cultrex in 100µl phenol free complete microglia …
• A Dimeric Rocaglate Promotes Multivalent eIF4A-RNA Assembly
  J Liu, MK Moore, K Lou, DR Wassarman, A Arab - … 2026
  Ligand dimerization represents a powerful strategy to enhance avidity, potency, and selectivity. Leveraging the natural-product molecular glue Rocaglamide (RocA), we …
• The generation of alternative transcripts as a means of regulating phosphorylation in sonic hedgehog medulloblastoma
  K Ogawa - 2025 2025
  Protein phosphorylation is critical in development and tumor progression; however, the promiscuity of kinases often limits the ability to regulate phosphorylation events …
• Youth-associated protein TIMP2 regulates microglial state and function in healthy and aged mice
  BM Hemmer, SM Philippi, AC Ferreira - Nature … 2026
  … Cells were plated on imaging plates (Cellvis, P96-1.5HN) or poly-L-Lysine-coated glass coverslips (Electron Microscopy Sciences, 72292-04) in DMEM supplemented …
• Understanding the Control Logic of Subcellular Self-Organization: From Ciliate Morphology to Biomolecular Condensates
  ZM Xu - 2026 2026
  … collini cells were seeded in a 96-well glass- bottom plate (Cellvis P96-1.5HN) at the … 96-well glass-bottom plates (Cellvis P96-1.5HN) and analyzed either as a detailed 3D …
• Stress-induced recruitment of small heat shock protein 1 at the outer mitochondrial membrane underlies resistance to apoptosis
  AK Mendes, SLM in't Groen, V De Winter - Cell Death … 2026
  … Cells were seeded in 96-well plates (P96-1.5HN, Cellvis, USA) with a concentration of 5 x 104 cells per well and treated with increasing concentrations of H₂O₂ (25–125 …
• Structural Mechanisms of DNAJC13 Dimeric Assembly and InsP6 binding in Recycling Endosome Regulation
  T Fu, C Lee, FV Hundley, JA Paulo, JW Harper - bioRxiv 2026
  … experiments were performed in 96-well glass bottom plates (Cellvis, P96-1.5HN). Wells were coated with 2 mg/mL BSA for 30 min and washed three times with PBS buffer (…
• A highly Ca2+-permeable channelrhodopsin from the ancyromonad Nutomonas longa enables optogenetic control of Ca2+ signaling
  EG Govorunova, Y Gou, AJ McDonald - bioRxiv 2026
  … For screening GEVIs, glass-bottom 640 96-well plates (P96-1.5HN, Cellvis) were first coated with poly-D-lysine (30-70 kDa) to 641 promote cell adherence to the glass. …
• An optogenetic smart microscopy platform reveals signaling dynamics-dependent control over collective cell migration
  HR Oatman, BC Lad, L Nguyen, JH Gubbens - Cell Systems 2026
  … Cell preparation for microscopy Cells were imaged on glass-bottomed, black-walled 96-well plates (Cellvis, P96-1.5HN) coated with fibronectin. Wells of 96-well plates …
• HiExM Enables Scalable Mapping of Organelle Morphology and Spatial Heterogeneity
  JH Day, JD Farrell, D Yang, FN Neira, EA Allen - bioRxiv 2026
  … CC-BY 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in …
• Adenine nucleotide translocase 2 (ANT2) deficiency reprograms ferroptosis in alveolar progenitor cells to promote emphysema
  U Mbaekwe, J Shi, NC Ting, Q Hu, S Gingras - bioRxiv 2026
  … alveolar organoids, isolated AT2 cells were plated in Matrigel (BD Biosciences, #354230) at 3,000 cells per dome in glass-bottom 96-well plates (Cellvis, Cat#: P96-1.5HN…
• Pathogenic DRP1 variants reveal a role for biomolecular condensation in mitochondrial fission
  KA Ross, AM Travis, MC Harwig, MS Young - bioRxiv 2026
  … A #1.5 high-performance cover glass 96-well plate (Cellvis; P96-1.5HN) or a Costar 96-well clear flat bottom plate (Corning; 3909) was used for each experiment. HEPES …
• Cytoplasmic LINE-1 Reverse Transcriptase Activity and Its Cellular Consequences
  L Kagermazova - 2026 2026
  Transposable elements–mobile genetic sequences capable of moving within genomes–make up nearly half of the human genome and have profoundly shaped its …
• A modular live-cell biosensor for extracellular protease activity
  B Ramm, M Weatherly, JE Toettcher - bioRxiv 2026
  … Cells were plated on fibronectin-coated, 96-well plates 651 with a #1.5 cover glass bottom (Cellvis, #P96-1.5HN). Plates were coated with 10 μg/ml 652 fibronectin diluted …
• Human macrophages encode stimulus-specific information of prior exposures through trained immunity
  A O'Farrell, Z Niu, J Li, LC Van Eyndhoven, K Sarma - Cell Systems 2026
  … seeded at a density of 800,000 cells per milliliter in Cellvis 24 well glass imaging plates (Fisher NC0397150) or Cellvis 96 well glass imaging plates (Fisher P96-1.5HN). …
• Cell wall remodeling is required for crowding homeostasis during cell growth in S. cerevisiae
  K Sakai, M Kunzi, F Uliana, L Willig, R Lappalainen - bioRxiv 2026
  The cytoplasm is a densely crowded environment that plays essential roles in intracellular functions. Its biophysical properties can be markedly altered in response to …
• G quadruplex DNA facilitates a pervasive path to homologous recombination
  BL Thakur, P Basak, S Khurana, S Sharma, YH Lai - bioRxiv 2026
  … , who has granted bioRxiv a license to display the preprint in perpetuity. It is made … (which was not certified by peer review) is the author/funder, who has granted bioRxiv a …
• LASER couples damage sensing to ESCRT assembly for lysosome repair
  CS Goul, A Jain, S Yitiz, ZE Soltani, S Yang, S Rapp - Nature 2026
  Lysosomal membrane integrity is essential for cell survival, but how damage sensing is spatiotemporally coupled to repair remains poorly understood. Recruitment and …
• A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo.
  S Yang, AJ McDonald, X Lu, V Villette - Biorxiv: the Preprint … 2026
  … For 620 screening GEVIs, glass-bottom 96-well plates (P96-1.5HN, Cellvis) were first coated with poly-D-lysine (30- 621 70 kDa) to promote cell adherence to the glass. …
• POCKET-seq enables genome-wide profiling of on-and off-target transcriptional regulation events by dCas9-KRAB during CRISPR interference experiments
  CM Joyce, GD Kramer, JT Vu, KU Tavasoli - bioRxiv 2026
  … Cells were plated on glass-bottom 96-well plates (Cat#P96-1.5HN; Cellvis) and grown until cell 215 confluency reached between 50% and 80% at the time of fixation. …
• An adaptable, self-organizing, single-cell morphology circuit optimizes suctorian predatory trap structure
  Z Xu, LE Mazurkiewicz, M Olivetta, M Morrissey - Current Biology 2026
  … a 96-well glass-bottom plate (Cellvis P96-1.5HN) and imaged as the pre-feeding time … collini cells were seeded in a 96-well glass-bottom plate (Cellvis P96-1.5HN) at the …
• A fusion Cell-Permeable C16orf74 Peptide Selectively Disrupts Calcineurin-NFAT Interaction and Inhibits T-cell Activation Without Cytotoxicity
  A Cohen, M Gabay, S Gupta, M Sova, D Bar, J Tubiana - bioRxiv 2026
  … Cells were seeded at 3,000 cells per well in a 96-well glass-bottom plate (Cellvis, P96-1.5HN) in culture media, 2 days before the experiment. On the day of the experiment…
• A Bioprinted Head and Neck Cancer Organoid-Based Platform for Evaluating Multimodal Therapies
  L Lin, KK Bommakanti, C Wooten, AE Gonzalez - bioRxiv 2026
  … (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this …
• ELDR-Glo, a biosensor for cell age and quiescence depth
  MS Johnson, S Kamath, D Fleifel, T Hill, L Mei, N Das - bioRxiv 2026
  … Before imaging RPE,MCF7s, or MCF10as, cells were seeded at low density on glass- 485 bottom plates (Cellvis Ca t#P96-1.5P) in appropriate imaging media (no phenol …
• Synaptic activity controls local exposure of an'eat-me'signal via ANO3-ITPR1 signaling
  K Shroff, MC O'Brien, TJ Smith, J Fang, KS Yu - bioRxiv 2026
  … CC-BY 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in …
• Ionic Exposure History Shapes Inner Nuclear Membrane Voltage and Chromatin Texture Responses
  H Sediqi, J Mathews, G De Nola, AKR Lytton-Jean - bioRxiv 2026
  … The cells were then cultured at 20 000 cells per well into a 96-well plate (Cellvis, Cat: P96-1.5P) 24 hours prior to imaging. Cells were then stained with Hoechst 33342 (1 …
• Hypoxia Inducible Factor 1a (Hif1a) and Hypoxia in Tendon Development and Disease
  S Steltzer - 2026 2026
  Tendons and ligaments are collagen-rich tissues required for the transmission of force from muscle to bone or bone to bone, respectively. During postnatal development, …
• Molecular and Structural Characterization Reveals Divergent Extracellular Vesicle Profiles Between Wild Type and Alzheimer's Disease Cerebrocortical Organoids.
  A Balistreri, N Turner, J Compher - Biorxiv: the Preprint … 2026
  … EV purification samples were diluted to 20 ug of protein in the wells of a 96 well plate 260 (P96-1.5P) with DPBS Ca2+ Mg2+ and 10 µM Thioflavin-T (ThT, EW-88226-62, …
• A TSST-1 structural motif disrupts endothelial programs required for vascular regeneration
  SS Tang, O Babatunde, PM Tran, XJ Wu - bioRxiv 2026
  Staphylococcus aureus causes profound vascular damage during infection, where it inflicts vascular injury across organs and generates lesions that fail to heal. …
• ATR enforcement of the S/G2 checkpoint prevents premature S phase shutdown and genome instability
  MJ McEvoy, JC Saldivar - bioRxiv 2026
  … siRNA Transfection Cells were seeded at 20,000 cells per well in glass-bottom 96-well plates (Cellvis P96-1.5P) and reverse-transfected with either non-targeting siRNA (…
• G2-to-G0 cell cycle exit underlies sensitivity to ATR inhibition via the p53-p21-RB1 axis
  C Sanchez, CO Marmolejo, J Lee, JC Saldivar - bioRxiv 2026
  Ataxia-telangiectasia and Rad3-related (ATR) is an essential DNA damage response kinase that protects genome integrity by controlling cell cycle checkpoints, regulating …
• ChemCell: Chemical Tethering of Large Biomolecules to Cell Surfaces through Diels-Alder Ligation
  R Dzijak, S Bellová, A Kovalová, V Šlachtová, M Rahm - bioRxiv 2026
  … For microscopic observations cells were seeded on a 96 well plate dish with a glass bottom (Cellvis, P96-1.5P). Upon treatment they were washed and DMEM medium …
• Rab12 is a regulator of mitophagy and mitochondrial homeostasis
  T Richbourg, A George, A Bitar, IT Ryde, C Farrell - … 2026
  … 96-well plates with a glass-like polymer coverslip bottom (Cellvis, P96–1.5P) were stamped using the Bravo Automated Liquid Handling Platform (Agilent Technologies) …
• Senescence-directed nanotherapy ameliorates fibrosis and overcomes immune exclusion in cancer
  C Hinterleitner, VJA Barthet, HV Goldberg, KC Vogt - … 2026
  … Cells were resuspended in DMEM supplemented with 10% FBS and 1% PenStrep and seeded at 0.01×106 into a seeded in a 96-well plate (Cellvis, P96–1.5P),that was …
• The Disordered Region of ASXL1 Acts as an Auto‐Regulator Through Condensation
  X Fang, Q Li, W Zhang - Advanced Science 2026
  … For drug screening, ASXL1-TR-mEGFP U2OS cells were seeded on 96 well glass bottom plates (Cellvis, P96-1.5P), and treated the following day with compounds from …
• Skin-derived α-synuclein strains from PD, DLB, and MSA induce distinct intracellular pathology and neurodegeneration
  A Raina, W Wang, JC Gonzalez, X Yan - Journal of Biological … 2026
  … Two hundred single cells were plated in 100 μl fresh growth medium per well of a 96-well plate (P96–1.5P, CellVis). Twenty colonies were selected based on growth and …
• Synergistic inhibition of CHK1 and MUS81 to combat replication stress resistance in high-risk neuroblastoma
  E Hilgert, C Van Neste, SL Bekaert, F Martens - Scientific reports 2025
  High-risk neuroblastoma is a pediatric tumor that originates from immature sympathetic neuroblasts and is characterized by a low mutational burden. Nevertheless, it …
• Intramolecular competition generates pulsatory protein activity shaped by light, temperature, and evolution
  ZD Huang, M Forson, W Benman, KH Gardner - … 2025
  … Cells were seeded into Cellvis 96 well plates (#P96–1.5P) coated with fibronectin (… was used in 96 well plates (#P96–1.5P CellVis) to prevent overheating from optoplate. …
• Extracellular microRNAs modulate human microglial function through TLR8
  H Weidling, E Motta, LD Kuhrt, C Krüger - Frontiers in … 2025
  Objective MicroRNAs (miRNAs) are abundantly expressed in the brain and are specifically dysregulated in central nervous system (CNS) diseases. They act as post-…
• Fluorogenic RNA Aptamer-Based Activity Quantification of T7 RNA Polymerase
  K Mimouni, W Qiu, H Zhou - Biochemistry
  … Once prepared, Broccoli IVT reactions were transferred to a black-edge and clear-bottom 384-well plate (Cellvis P384-1.5HN) and monitored using a Neo2 plate reader (…
• Advanced Open-source Experimental-Design Tools for Microplate-Based Assays with Acoustic Liquid Handling
  VM Kattunga, SA Wrobel, CA Lerner, VM Derycz - bioRxiv 2026
  … All other ADE happened into 384-well glass-bottomed plates (Cellvis #P384-1.5HN). For manual culturing of dispersed human pancreatic β-cells, glass-bottomed 96-well …
• Opposing roles of serine and charge in IDR condensate miscibility
  G Pei, X Wang, X Quan, D Geng, Z Chen, W Xu - Nature Chemical … 2026
  Numerous biomolecular condensates coexist within cells, yet the factors governing their miscibility remain poorly understood. Here, by examining 28 intrinsically …
• Efficient Generation of Functional TCRαβ+ Cytotoxic T Cells from hiPSCs via Small-Molecule Modulation
  C Kubaczka, N Kambli, R Windisch, K Yu, Y Zhao - … 2026
  … Rows AO of a glass bottom 384-well plate (Cellvis, #P384–1.5HN) were coated with DLL4 (Thermo Fisher) and VCAM1 by adding 25 μl/well with a 300 μl 12-channel …
• A calcium imaging pipeline to detect and quantify compound-specific effects in human and mouse astrocytes and astrocyte-neuron cocultures
  J Krohn, L Breuer, S Wegmann, C Dean - bioRxiv 2026
  … effects in human and mouse astrocytes and astrocyte-neuron cocultures | bioRxiv … , 200 µL culture medium; 384-well (Cellvis, P384-1.5HN): ∼120,000 cells/cm², 100 µL …
• The Spatiotemporal Proteome Landscape of Aging: Structural determinants of age-sensitive proteome remodeling
  S Yoo, L Vannur, L Li, C Young, Q Liu, ZT Wen - bioRxiv 2026
  … Cells were imaged in 384-well glass-bottom plates (Cellvis, P384-1.5HN, USA). Images were 815 acquired on a Nikon CSU-W1 SoRa spinning-disk confocal microscope …
• Single-Cell Spatial Proteomics Uncovers Molecular Interconnectivity among Hallmarks of Aging
  S Yoo, C Young, L Li, L Vannur, J Zhuang, F Zheng - bioRxiv 2026
  Aging is accompanied by conserved hallmarks including genomic instability, epigenetic alterations, loss of proteostasis, and mitochondrial dysfunction, but how these …
• Revealing site-specific molecular features mediating contacts of heterochromatin protein 1 α (HP1α) and the interactions with chromatin and DNA in LLPS …
  Z Zhai, A Soman, J Kuang, J Guo, Y Chen - Communications … 2025
  … The 384-Well Microscopy Plates (Cellvis, #P384-1.5HN) were washed and then pre-treated with 1 M NaOH and 5 K mPEG-silane as described in one of our recent studies…
• 2050-084X
  L Adèle - eLife
  … P384-1.5HN) were washed with PBS and then permeabilized with 0.1% Triton X-100 (Sigma, Cat. No. T9284-500ML) solution in PBS for 15 minutes and again washed …
• Liquid-liquid phase separation of ATXN2L enhances mRNA translation in hepatocellular carcinoma
  S Wang, P Guo, W Ma, H Huang, Q Zhang - Cell Reports 2025
  … The samples were then transferred into a 386-well plate (P384-1.5HN, CellVis, Mountain View, USA) for observation. Imaging of the phase-separated structures was …
• Explainable AI to unveil cellular autophagy dynamics
  O Presacan, M Hernández Mesa, AC Aldea - Plos one 2025
  … Cells were imaged in a 384-well glass-bottom plate (Cellvis, P384-1.5HN) pre-coated with 0.5 mg/mL concanavalin A (Sigma, L7647). Live-cell time-lapse imaging was …
• Automated FLIM-FRET Segmentation Within RNP Condensates
  ND Powell, JM Marcus, LE Fahim, JE Lee - Bio-protocol 2026
  Ribonucleoprotein (RNP) condensates are membraneless organelles that exist alongside many RNA-driven processes, such as transcription and splicing. Despite their …
• Revealing the spatiotemporal dynamics of methionine metabolism with a genetically encoded single-fluorophore biosensor
  KA Cohen, A Jhawar, G Garcia, N Goni, M Chen - bioRxiv 2026
  … (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this …
• CRISPR/Cas9-mediated PHOX2B functional knock-out in IMR32 neuroblastoma cells impairs neuronal excitability through dysregulation of ion channels genes
  S Cardani, M Bertocchi, E Donà, F Chiesa - Frontiers in … 2026
  Introduction PHOX2B is a master transcriptional regulator of autonomic nervous system development whose mutations cause neurocristopathies including neuroblastoma …
• α-Synuclein triggers intercellular nanotubes formation to prevent apoptosis in astroglia by promoting stemness
  R Kashayap, A Sreenivas BK, V MR, RR Mundada - bioRxiv 2026
  Astrocytes play a significant role in neuroprotection by internalizing neurodegenerative aggregates and facilitating their degradation. Recent studies indicate that α-…
• Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes
  JV Bogdanović, J Galeota-Sprung, AS Kainth - bioRxiv 2026
  … (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this …
• Biomechanical Isolation is Required for Maintenance of the Cardiac Pacemaker Cell Fate
  M Bressan, A Laidman, T Henley, W Shi, K Scherrer - 2026 2026
  … Explants were placed in 35mm glass bottom dishes (Cellvis, D35-14-1.5-N) for … To prepare the 35 mm tissue culture dishes (Matsunami Glass, D35-14-0-U), the dishes …
• Probing RNA-protein interactions in the early mouse embryo
  P Bora, OJ Rando - bioRxiv 2026
  … mounted with VECTASHIELD mounting medium containing DAPI (Vector Laboratories H-1200), placed on glass-bottomed 35 mm dishes (Avantor/Matsunami D35-14-1-U)…
• Coordinated phase separation and phase transition underlie synaptic ribbon condensate formation and plasticity
  Y Liu, X Wang, T Zheng, F Niu, R Sun, C Yang, S Xu - bioRxiv 2026
  … For the CLEM experiment, HeLa cells were cultured on gridded glass-bottom dishes (D35-14- 535 1.5GI, Cellvis) and transfected with mCherry-RIBEYE_∆IDR. …
• Scaffold protein SHANK3 regulates endothelial cell motility and tissue mechanics
  MR Chastney, A Pink, J Härkönen, G Follain, V Stüve - bioRxiv 2026
  … For hydrogels (∼10 kPa), 35 mm glass bottom dishes (D35-14-1, CellVis) were incubated with 1 ml bind 638 silane solution (7.14 % Plus One Bind silane (Sigma) and …
• Direct Membrane Penetration of Oligoarginines by Fluorescence and Cryo-electron Microscopy Combined with Molecular Simulations
  KL Baxová, MI Morandi, N Scher, P Kula, O Tichacek - bioRxiv 2026
  … The grids were left in a tissue culture hood under UV for 1 hour before being transferred into a 14 mm glass-bottomed well of a 35 mm imaging plate (D35-14-1.5N, Cellvis)…
• Denatured Albumin Gains a Function of Regulating Platelet Activity
  V Pandey, S Kundu, SJ Wang, A Pyne, L Que, X Wang - bioRxiv 2026
  … 200 µL casein solution was added to the glass surface of a glass-bottomed Petri-dish (D35-14-1.5-N, Cellvis) which was subsequently incubated for 30 minutes at room …
• Steric shielding of the KRAS4B hypervariable region enables isoform-specific inhibition of prenylation
  JN Maskos, Y Stark, VL Rohner, A Häfliger - bioRxiv 2026
  … The cultures were maintained at 37 C with 5% CO₂ in an imaging dish with a glass bottom (D35-14-1.5-N, Cellvis). One hour before injection, the medium was replaced …
• Mechanical Signaling Drives Tunneling Nanotubes to Preserve Cytoskeleton Tension and Lamin Integrity Against α-Synuclein-Induced Senescence in Astroglia
  S Chatterjee, A Ravula, A Sreenivas BK, A Raghavan - bioRxiv 2026
  … https://doi.org/10.64898/2026.03.13.711517 doi: bioRxiv preprint … Glass-bottom 35 mm dishes (Cellvis, D35-14-1.5-N) were employed for certain experiments. …
• Red-Shifted Chemigenetic Glutamate Indicators for Recording Multiplexed Neural Computation
  N Tjahjono, JY Min, JA Chouinard - Available at SSRN …
  Neural computation relies on the integration of neurotransmitters, neuromodulators, and intracellular signal transduction. However, direct measurement of these …
• Digest before Ingest: Early Recruitment of Membrane-bound DNaseX to Phagocytic Cups in Macrophages
  A Pyne, V Pandey, S Kundu, S Ikegami, X Wang - bioRxiv 2026
  … 400 µL bacterial solution was incubated on glass bottom 475 petridish (D35-14-1.5-N, Cellvis) at room temperature condition for 1-3 days. The biofilm thickness depends …
• An ER retention motif controls the heteromeric stoichiometry of hERG1a/1b channels
  S Kannan, LR Ernandez, GA Robertson - bioRxiv 2026
  … The complete version history of this preprint is available at bioRxiv. … in 35mm glass bottom dishes with #1.5 cover glass (Cellvis, D35-14-1.5-N) using DMEM culture media (…
• Preparation of samples for Lipid-CLEM imaging
  A Nadler, HM Lennartz - 2026 2026
  … Under the cell culture hood: Place Sapphire disks in 35 mm glass bottom dishes Cellvis, #D35141.5N) and pre-incubate with cell culture medium for 30 min at 37 C. …
• Tissue-scale mechanics controls differentiation strategy and dynamics of epithelial multilayering
  C Villeneuve, SAE Hassikpezi, M Albu, M Rübsam - bioRxiv 2026
  Generating and maintaining multilayered epithelia requires coordinated cell division, differentiation, and tissue architecture, yet the precise mechanisms of multilayering …
• TP53/TAU axis regulates microtubule bundling to control alveolar stem cell–mediated regeneration
  S Konishi, K Enkhbayar, S Liu, N Miyashita - The Journal of Clinical … 2026
  … suspensions of mouse AT2s were resuspended in SFFF containing lentivirus at 1:100 and seeded on a fibronectin-coated, glass-bottom dish (Matsunami Glass, D35-14-1-…
• Acute in vivo proximity labeling for membrane targeted proteomics in neuronal circuits
  M Anguiano, R Zhang, M Robles, KP Adams - bioRxiv 2025
  … Neurons were plated on treated 35 mm glass bottom dishes (D35-14-1-N, Cellvis) and maintained at 37C, 5% CO2. The culture media was replaced 24 hr after the plating …
• NOP16 Sequesters EED to Nucleolus to Suppress Histone H3K27me3 and Promote Breast Cancer
  K Takashima, A Khan, EL Greer - Available at SSRN 7162927
  Post-translational modifications of histone tails alter chromatin accessibility to regulate gene expression. Some viruses exploit the importance of histone modifications by …
• Novel and Diverse Mycoviruses in Hypovirulent Botrytis cinerea Isolates Infecting Raspberries and Strawberries
  SC Drury, MA Poursalavati, MP Lemoyne, D Xu - 2026 2026
  Botrytis cinerea causes significant economic losses in many crops, including vegetables, fruits, and ornamental plants, and its management is becoming increasingly …
• Pyoderma gangrenosum caused by the molecular uncoupling of OTULIN catalytic activity and LUBAC binding
  B Swaminathan, HM Gil, S Bhattad, J Janardhanan - Nature … 2026
  The pathogenic mechanisms underlying pyoderma gangrenosum (PG) remain unclear. Here we report three patients with PG from two unrelated kindreds with …
• Multimodal atlas of human atherosclerosis links granular vascular cell states to coronary artery disease risk
  JV Mosquera, I Tang, M Murach, G Auguste, A Kodali - medRxiv 2026
  Advances in single-cell and spatial assays have revolutionized the scale and resolution of molecular tissue profiling. Here we present MetaPlaq, a multimodal atlas of …
• Genetic suppression of myeloid receptor Clec7a attenuates microglia neuroinflammation and promotes microglial phagocytosis to delay disease progression in ALS …
  X Chen, H Yan, H Wei, S Sajadi, J Hu, V Vasconcellos - bioRxiv 2026
  … was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint …
• DNAharvester: A Nextflow Pipeline for Analysing Highly Degraded DNA from Ancient and Historical Specimens
  B Sharif, VE Kutschera, N Oskolkov, B Guinet, E Lord - bioRxiv 2026
  … CC-BY-NC-ND 4.0 International license made available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display …
• Regulatory elements in the Sox9 locus license the initiation of pancreatic ductal adenocarcinoma
  M Ballester, A Kurilla, Y Dai, L Bergara-Muguruza - Cell Reports 2026
  Cellular plasticity enables tissue regeneration but can be hijacked by oncogenic programs. In the pancreas, Kras acts on tissue-specific enhancers to lock regeneration …
• Insights into spacer acquisition of the type VA CRISPR–Cas system of Francisella novicida U112
  F Hille, C Wang, K Finstermeier, D Beerens - Nucleic Acids … 2026
  CRISPR–Cas systems immunize prokaryotic cells through a CRISPR adaptation process, in which short DNA fragments from foreign elements are acquired and integrated …
• Pediatric acute myeloid leukemia tumor composition predicts patient outcomes at diagnosis and reveals mechanisms of resistance to chemotherapy
  P Sumazin, M NajafPanah, A Stevens, M Redell - 2026 2026
  Although most pediatric acute myeloid leukemia (pAML) patients achieve complete remission with standard-of-care chemotherapy, overall outcomes are poor, and 40% …
• PARG regulates the proteasomal degradation of TARG1
  J Groslambert, SC Buch-Larsen, IA Hendriks - Cell reports 2026
  ADP-ribosylation (ADPr) is a reversible modification of macromolecules critical for the regulation of genome stability, stress responses, and proteostasis. While the roles of …
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  LT Fontanals, JML Bayer - diposit.ub.edu
  Molecular Characterization and Novel Therapeutic Approaches in Hepatocellular Carcinoma Laura Torrens Fontanals Aquesta tesi doctoral està subjecta a la llicència …
• Natural variation in the GhTTL promoter modulates cotton fiber elongation through the GhTALE–GhTTL–GhBIN2 module
  L Liu, Z Liu, L Fan, M Yu, G Qanmber, L Wang, Y Li - Plant … 2025
  In cotton, fiber length is a key determinant of industrial utility and is one of the most important agricultural traits shaped during domestication. However, the genetic …
• Dual inhibition of FAS and HAS2/3 by 4-MU in Realgar-Coptis chinensis unveils a metabolic checkpoint for liver cancer therapy
  S Wu, Y Wang, D Deng, G Zheng, H Mei - Natural Products and … 2025
  Modern pharmacology has found that both Realgar and Coptis chinensis can induce apoptosis in tumor cells, and traditional Chinese medicine theory suggests the …